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From the Next Generation Technical Manual, p.67: "As used aboard the USS Enterprise, antimatter is first generated at major Starfleet fueling facilities by combined solar-fusion charge reversal devices, which process proton and neutron beams into antideuterons, and are joined by a positron beam accelerator to produce antihydrogen (specifically antideuterium). Even with the added solar dynamo input, there is a net energy loss of 24% using this process, but this loss is deemed acceptable by Starfleet to conduct distant interstellar operations.

p.71 of the same manual details onboard antimatter generation processes using the Bussard collectors, but warns that this process is wildly power-intensive and inefficient, and is only used in situations of absolute emergency.

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